製品の概要

  • 製品名Human CXCL11 ELISA Kit (I-TAC)
    CXCL11 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.5 pg/ml - 600 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 92.82 80% - 105%
    Serum 95.28 84% - 106%
    Plasma 93.47 83% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human CXCL11 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL11 in serum, plasma (heparin or EDTA), and cell culture supernatants.

    This assay employs an antibody specific for Human CXCL11 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL11 C bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • 組織特異性High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • b R1
    • Beta R1
    • Beta-R1
    • betaR1
    • C X C motif chemokine 11
    • C-X-C motif chemokine 11
    • Chemokine (C-X-C motif) ligand 11
    • Chemokine C-X-C motif ligand 11
    • CXCL11
    • CXL11_HUMAN
    • H174
    • I TAC
    • I-TAC
    • Interferon gamma inducible protein 9
    • Interferon gamma-inducible protein 9
    • Interferon inducible T cell a chemoattractant I TAC
    • Interferon inducible T cell alpha chemoattractant
    • Interferon-inducible T-cell alpha chemoattractant
    • IP 9
    • IP-9
    • IP9
    • ITAC
    • MGC102770
    • SCYB11
    • SCYB9B
    • Small inducible cytokine B11
    • Small inducible cytokine subfamily B (Cys X Cys) member 11
    • Small inducible cytokine subfamily B (Cys X Cys) member 9B
    • Small inducible cytokine subfamily B, member 11
    • Small inducible cytokine subfamily B, member 9B
    • Small-inducible cytokine B11
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CXCL11 ELISA Kit (I-TAC) 画像

  • Representative Standard Curve using ab100580.

  • Representative Standard Curve using ab100580.

プロトコール

Human CXCL11 ELISA Kit (I-TAC) (ab100580) 使用論文

This product has been referenced in:
  • Asemota OO  et al. Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians. N Am J Med Sci 6:84-8 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24695523) »

See 1 Publication for this product

Product Wall

I-TAC-CXCL11 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I-TAC-CXCL11 secretion by human macrophages isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr, good specificity as shown by higher secretion by M1 macrophages (MSCF+IFNg) on day 7 compared to the other time point and macrophage subtypes (M0=MCSF M2a=MCSF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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投稿 Apr 09 2014

Veuillez trouver ci-après la réponse que j'ai obtenu du labo concernant le problème rencontré avec les kits ELISA ab100580 et ab100506. Laréponse est en anglais, j'espère que cela ne vousdérange pas. Tha...

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Merci de m'avoir envoyé le fichier Excel avec vos résultats. J'ai noté que seul l'axe absices (concentrations pg/ml) était logarythmique, mais pas l'axe des DO à 450nm. pourriez-vous s'il vous plait refaire et m'envo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"