Human Cu/Zn Superoxide Dismutase ELISA Kit (ab119520)

製品の概要

  • 製品名Human Cu/Zn Superoxide Dismutase ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5.8%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.04 ng/ml
  • 検出範囲
    0.08 ng/ml - 5 ng/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 98 89% - 108%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Cu/Zn Superoxide Dismutase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.

    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • 特記事項

    ab119520 Cu/Zn Superoxide Dismutase Human ELISA kit has been shown to detect both SOD1 and SOD3.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 配列類似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻訳後修飾Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 細胞内局在Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 別名
    • ALS
    • ALS1
    • Cu/Zn superoxide dismutase
    • homodimer
    • hSod1
    • Indophenoloxidase A
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • SOD3
    • SODC_HUMAN
    • Superoxide dismutase [Cu-Zn]
    • Superoxide dismutase 1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase 3
    • Superoxide dismutase cystolic
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Cu/Zn Superoxide Dismutase ELISA Kit 画像

  • Representative Standard Curve using ab119520.

プロトコール

Human Cu/Zn Superoxide Dismutase ELISA Kit (ab119520) 使用論文

ab119520 has not yet been referenced specifically in any publications.

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Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with sampl...

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Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: http://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: http...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"