製品の概要

  • 製品名Human Complement C4 ELISA Kit
    Complement C4 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.3%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.07 ng/ml
  • 検出範囲
    0.078 ng/ml - 20 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Complement C4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in urine, milk, saliva, CSF and cell culture supernatants.

    A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C4 captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 組織特異性Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response.
  • 関連疾患Complement component 4A deficiency
    Systemic lupus erythematosus
  • 配列類似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Acidic complement C4
    • Basic complement C4
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
    • C4A
    • C4B
    • CO4
    • CO4A_HUMAN
    • Complement C4 A
    • Complement C4 B
    • Complement C4 gamma chain
    • Complement component 4A
    • Complement component 4B
    • CPAMD2
    • CPAMD3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Complement C4 ELISA Kit 画像

  • Representative Standard Curve using ab108825

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Human Complement C4 ELISA Kit (ab108825) 使用論文

ab108825 has not yet been referenced specifically in any publications.

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