製品の概要

  • 製品名
    Human Complement C3 ELISA Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 10%
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.3 ng/ml
  • 検出範囲
    0.625 ng/ml - 40 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Complement C3 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.

    A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C3 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C3 Microplate (12 x 8 well strips) 1 unit
    Complement C3 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 機能
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 組織特異性
    Plasma.
  • 関連疾患
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 配列類似性
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Acylation stimulating protein cleavage product
    • AHUS5
    • ARMD9
    • ASP
    • C3
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • CO3_HUMAN
    • Complement C3
    • Complement C3c alpha'' chain fragment 2
    • Complement component 3
    • Complement factor 3
    • CPAMD1
    • HEL S 62p
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Complement C3 measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108823

プロトコール

参考文献

This product has been referenced in:
  • McGuire JL  et al. The complement system, neuronal injury, and cognitive function in horizontally-acquired HIV-infected youth. J Neurovirol 22:823-830 (2016). ELISA ; Human . Read more (PubMed: 27273074) »

See 1 Publication for this product

レビューと Q&A

The first assay you asked about, ab108823, is that kind of assay (sandwich), but the secondary (detection) antibody is biotinylated, not conjugated to an enzyme. The enzyme (HRP) is conjugated to streptavidin, which binds to the biotin on the antibody....

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A better choice for plasma samples is ab108822.


Complement C3 is very abundant in plasma/serum samples. We have developed two different assays.

ab108823 is a sensitive sandwich ELISA assay. If you use this kit for plasma or serum sample, it requires very large dilution. Normal plasma C...

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Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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I am sorry to hear that you have experienced so many problems with the Complement C3 Human ELISA kit (ab108823). I appreciate the time you took to provide further details.

After reviewing the provided information I suggest to verify the sample...

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Thank you for contacting us.

I have been in touch with the lab to inquire aboout use ofab108823 Complement C3 Human ELISA kit with plasma.This kit can be used for plasma, but you need to find out the appropriate dilution factor. We suggest...

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Muchas gracias por tu respuesta.

Actualmente tenemos una promoción para España por la que se aplica el 25% de descuento a todos los productos que no son anticuerpos primarios. La información sobre la promoción est&...

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Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

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Gracias por contactar con abcam.

Me gustaría informarle que tenemos 2 kits para la deteccion de humano C3 (tambien llamado ASP).

Las referencias sonab108822 (para Serum, Plasma) and ab108823 (para Cell culture supernatant, Sa...

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Thank you for contacting us.

Unfortunately, the lab does not have any information regarding the isoelectric point for this kit. I am sorry I could not be more helpful, please let me know if you need any additional information.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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