製品の概要

  • 製品名Human CNTF ELISA Kit
    CNTF キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.2 ng/ml
  • 検出範囲
    0.823 ng/ml - 200 ng/ml
  • 添加回収試験

    > 92 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.45 84% - 104%
    Serum 92.35 82% - 102%
    Plasma 93.28 83% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
    非交差種: Rat
  • 製品の概要

    Abcam’s CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in serum, plasma (Human CNTF concentration is pretty low in normal serum/plasma, it may not be detected in this assay), and cell culture supernatants.

    This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • 細胞内局在Cytoplasmic
  • 別名
    • AI429687
    • Ciliary Neurotrophic Factor
    • HCNTF
    • MGC41235
    • OTTHUMP00000174731
    • OTTMUSP00000029848
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CNTF ELISA Kit 画像

  • Representative Standard Curve using ab99994

  • Representative Standard Curve using ab99994

プロトコール

Human CNTF ELISA Kit (ab99994) 使用論文

ab99994 has not yet been referenced specifically in any publications.

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Thank you for your inquiry. For the kit ab99994, the capture antibody was collected from mouse ascities after the mouse was injected with the immunogen (so the host is mouse). The host for the detection antibody is goa...

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Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins ar...

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I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code i...

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Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection ...

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Thank you for contacting Abcam regarding ab99994. I have received some useful information from the lab regarding the detection of CNTF is plasma samples. CNTF is a fairly low abundant protein in human serum and plasma and therefore may be difficult...

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Thank you for calling Abcam. Regarding the preparation of plasma for use with this kit, please see the following protocol: - Collect blood into EDTA or Sodium heparin tube - Spin 10 minutes at 3000 rpm - Aliquot into small tubes and store at –...

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