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Abcam’s anti-Chlamydia trachomatis IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Chlamydia trachomatis in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia trachomatis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Chlamydia trachomatis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia trachomatis IgM sample captured in plate.
|内容||ラベル||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Chlamydia trachomatis (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Chlamydia trachomatis anti-IgM HRP Conjugate||Black cap||1 x 20ml|
|Chlamydia trachomatis IgM Cut-off Control||Green cap||1 x 3ml|
|Chlamydia trachomatis IgM Negative Control||Blue cap||1 x 2ml|
|Chlamydia trachomatis IgM Positive Control||Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgM Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108722 has not yet been referenced specifically in any publications.