Human Anti-Chlamydia trachomatis IgA ELISA Kit (ab108718)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Chlamydia trachomatis IgA ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Pos. Serum 24 4.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Pos. Serum 12 7.1% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Chlamydia trachomatis IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Chlamydia trachomatis in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia trachomatis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Chlamydia trachomatis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia trachomatis IgA sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia trachomatis (IgA) Coated Microplate (12 x 8 wells) 1 unit Chlamydia trachomatis anti-IgA HRP Conjugate Violet Bottle, Black Cap 1 x 20ml Chlamydia trachomatis IgA Cut-off Control Yellow Bottle, Green Cap 1 x 3ml Chlamydia trachomatis IgA Negative Control Yellow Bottle, Blue Cap 1 x 2ml Chlamydia trachomatis IgA Positive Control Yellow bottle, Red Cap 1 x 2ml Cover foil 1 unit IgA Sample Diluent White Cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
画像
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108718 は 1 報の論文で使用されています。
- Khan KN et al. Decreased occurrence of endometriosis in women with Chlamydia trachomatis infection. Am J Reprod Immunol 86:e13498 (2021). PubMed: 34523177