製品の概要

製品の詳細

  • 由来Synthetic

特性

Our Abpromise guarantee covers the use of ab37237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • CDS 1
    • Cds1
    • Cds1 homolog
    • Checkpoint kinase 2
    • Checkpoint like protein CHK2
    • CHEK 2
    • Chek2
    • Chk 2
    • CHK2 checkpoint homolog
    • CHK2 checkpoint homolog (S. pombe)
    • CHK2_HUMAN
    • hCds1
    • HuCds 1
    • LFS 2
    • LFS2
    • PP1425
    • RAD 53
    • RAD53
    • Rad53 homolog
    • Serine/threonine protein kinase Chk2
    • Serine/threonine-protein kinase Chk2
    see all
  • 機能Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • 組織特異性High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • 関連疾患Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated by PLK4.
  • 細胞内局在Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

Human Chk2 peptide 画像

  • All lanes : Anti-Chk2 (phospho T26) antibody (ab26338) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Gamma-Irradiated HeLa Lysate - 4 hour exposure at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human Chk2 (phospho T26) peptide (ab27902) at 1 µg/ml
    Lane 4 : Gamma-Irradiated HeLa Lysate - 4 hour exposure at 20 µg with Human Chk2 (phospho T26) peptide (ab27902) at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human Chk2 peptide (ab37237) at 1 µg/ml
    Lane 6 : Gamma-Irradiated HeLa Lysate - 4 hour exposure at 20 µg with Human Chk2 peptide (ab37237) at 1 µg/ml

    Secondary
    Lanes 2 - 6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Human Chk2 peptide (ab37237) 使用論文

ab37237 has not yet been referenced specifically in any publications.

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