Human cervix tumor lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 49 yrs) (ab43897)

製品の概要

  • 製品名Human cervix tumor lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 49 yrs)
    Cervix ライゼート 製品一覧
  • 製品の詳細
    Human cervix insoluble fraction
  • 特記事項

    Tissue: Cervix uteri Location: Cervix Diagnosis: Adenosquamous cell cervical carcinoma, moderately differentiated Stage: I T1b2NXM0 Grade: II Appearance: Macroscopic: Organ: Cervix Size: 2.0 x 2.5 cm Color: Pink Consistency: Firm Cut surface: Pink-gray Characteristics: +/- Encapsulated: - Invaded: + Hemorrhagic: + Cystic degeneration: - Calcification: - Histologic pattern: Cell distribution: +/- Diffuse: + Mosaic: - Necrosis: + Lymphocytic infiltration: + Vascular invasion: + Clusterized: - Alveolar formation: + Indian file: - Structure/Pattern: +/- Streaming: - Storiform: + Fibrosis: + Pallisading: - Cystic degeneration: - Bleeding: + Myxoid change: + Psammoma body: - Cellular differentiation: Squamous: +/- Squamoid: + Spindle: + Keratin: + Desmosome: - Pearl : - Adenomatous: +/- Glandular cell: + Cell stratification: - Secretion: + Intracellular vacuole: - Glandular formation: + Sarcomatous: +/- Round cell: - Spindle cell: - Leiomyoblast: - Lipoblast: - Rhadomyoblast: - Nuclear atypia: Nuclear appearance: 0 I II III Anisonucleosis: II Hyperchromatism: II Nucleolar prominent: I Multinucleated giant cell: II Mitotic activity: II Nuclear grade: II.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43897: Extraction 1: soluble protein fraction ab43895 Human Cervix Tumor Tissue lysate ab43894 Human Cervix Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43897 Human Cervix Tumor Tissue lysate ab43896 Human Cervix Normal Tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 0.1% SDS, PBS, 5M Urea, 2M Thiourea, 50mM DTT, pH 7.4

    Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human cervix tumor lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 49 yrs) (ab43897) 使用論文

    ab43897 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"