製品の概要

  • 製品名Human Ceruloplasmin ELISA Kit
    Ceruloplasmin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.95 ng/ml
  • 検出範囲
    1.25 ng/ml - 320 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Ceruloplasmin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin concentrations in urine, saliva, milk and culture supernatants.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 配列類似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Ceruloplasmin ELISA Kit 画像

  • CERP measured in culture supernatants (duplicates +/- SD). Samples were diluted in the range of 1/1-1/30.

  • Representative Standard Curve using ab108818

プロトコール

Human Ceruloplasmin ELISA Kit (ab108818) 使用論文

This product has been referenced in:
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »

See 1 Publication for this product

Product Wall

Thank you for your phone call this morning.

I have now received the confirmation from the laboratory that in the standard vial you received is indeed an amount of 320ng.

I apologize again for this missing information.

Plea...

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Thank you for contacting us. The standard is 320 ng to be mixed with 1 ml diluent to create the 320 ng/ml solution.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your patience while I contacted the lab. They have informed my that ab108818, would not be suitable for serum samples and that it only has around 20% cross-reactivity with monkey samples. However, ab108817, will react with...

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