製品の概要

  • 製品名Human CEACAM1 ELISA Kit (BGP)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    20.58 pg/ml - 15000 pg/ml
  • 添加回収試験

    > 83 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 83.49 75% - 90%
    Serum 96.92 82% - 112%
    Plasma 86.5 70% - 105%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CEACAM1 (BGP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEACAM1 in serum, plasma, cell culture supernatant.

    This assay employs an antibody specific for Human CEACAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CEACAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEACAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEACAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
  • 別名
    • Antigen CD66
    • BGP
    • BGP 1
    • BGP1
    • BGPI
    • Biliary glycoprotein
    • Biliary glycoprotein 1
    • Biliary glycoprotein adhesion molecule
    • Carcinoembryonic antigen related cell adhesion molecule 1
    • CD66a
    • CEA related cell adhesion molecule
    • CEACAM 1
    • CEACAM1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CEACAM1 ELISA Kit (BGP) 画像

  • Representative Standard Curve using ab99993.

  • Representative Standard Curve using ab99993.

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Human CEACAM1 ELISA Kit (BGP) (ab99993) 使用論文

ab99993 has not yet been referenced specifically in any publications.

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