製品の概要

  • 製品名Human CD95 ELISA Kit (TNFRSF6)
    CD95 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 14 1719pg/ml 106 6.1%
    Sample B 16 703pg/ml 39 5.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 1752pg/ml 145 8.2%
    Sample B 8 856pg/ml 68 7.9%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 47 pg/ml
  • 検出範囲
    93.75 pg/ml - 3000 pg/ml
  • 全工程の試験時間
    1h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human soluble CD95 (TNFRSF6) ELISA is to be used for the in-vitro quantitative determination of soluble CD95 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD95 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD95 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD95 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD95 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD95.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 組織特異性Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 関連疾患Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • ドメインContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • ALPS1A
    • APO 1
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Fas
    • Fas AMA
    • Fas antigen
    • FAS1
    • FASLG receptor
    • FASTM
    • TNF receptor superfamily member 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD95 ELISA Kit (TNFRSF6) 画像

  • Representative Standard Curve using ab45922

プロトコール

Human CD95 ELISA Kit (TNFRSF6) (ab45922) 使用論文

ab45922 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

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These ELISA kits are from two different sources but they are similar.

I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ...

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Thank for your quick response. As I have noted in my previous e-mail, the kit seems to be working since the standard curve obtained is fine. The actual standard values are concentration-dependent and the curve is linear. We assayed over 50 health...

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Thank for your enquiry regarding ab45922 and for providing some data saved in Excel file. After reviewing the results you have kindly sent to us, the standard curve obtained is fair and it seems the kit worked fine. Could you please confirm t...

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