製品の概要

  • 製品名
    Human CD86 ELISA Kit
    CD86 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 37.25U/ml 0.329 0.88%
    Sample B 8 5.09U/ml 0.193 3.79%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 39 37.63U/ml 0.611 1.62%
    Sample B 39 4.92U/ml 0.348 7.06%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.6 U/ml
  • 検出範囲
    1.187 U/ml - 38 U/ml
  • 全工程の試験時間
    2h 40m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human CD86 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD86 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD86 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD86.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • CD86 in U937 lysates from control cells or cells stimulated for 24 hours with 50 ng x mL-1 PMA (ab120297) or PMA with 1 ug x mL-1 LPS for the last 6 hours (duplicates; +/- SD).

    2e6 snap frozen cells were lysed in 0.3 mL RIPA lysis buffer on ice, sonicated in an ice cold water bath for 12 intervals for a total of 3.5 minutes. The supernatants were cleared by centrifugation (max speed, 10 minutes, 4 degrees), and tested undiluted or diluted 1/4.

  • CD86 measured in undiluted 72 hours culture supernatants from control of treated (5 ug x mL-1 PHA; Sigma) PBMCs (1e6 cells x mL-1; duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab45921

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参考文献

ab45921 has not yet been referenced specifically in any publications.

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