製品の概要

  • 製品名Human CD86 ELISA Kit
    CD86 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 37.25U/ml 0.329 0.88%
    Sample B 8 5.09U/ml 0.193 3.79%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 39 37.63U/ml 0.611 1.62%
    Sample B 39 4.92U/ml 0.348 7.06%
  • テスト
    2 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.6 U/ml
  • 検出範囲
    1.187 U/ml - 38 U/ml
  • 全工程の試験時間
    2h 40m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human CD86 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD86 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD86 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD86.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • 組織特異性Expressed by activated B-lymphocytes and monocytes.
  • 配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • Activation B7-2 antigen
    • Activation B7-2 antigen 3
    • B-lymphocyte activation antigen B7-2
    • B-lymphocyte activation antigen B7-2 2
    • B7
    • B7 2
    • B7-2
    • B7.2
    • B70
    • B72 antigen
    • BU63
    • CD28 antigen ligand 2
    • CD28 antigen ligand 2 2
    • Cd28l2
    • CD28LG2
    • CD86
    • CD86 antigen
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2
    • CD86 molecule
    • CD86_HUMAN
    • CLS1
    • CTLA-4 counter-receptor B7.2
    • CTLA-4 counter-receptor B7.2 2, 3
    • Early T-cell costimulatory molecule 1
    • ETC-1
    • FUN 1
    • FUN-1
    • LAB72
    • Ly-58
    • Ly58
    • MB7
    • MB7-2
    • Membrane glycoprotein
    • MGC34413
    • T-lymphocyte activation antigen CD86
    • TS/A-2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD86 ELISA Kit 画像

  • CD86 in U937 lysates from control cells or cells stimulated for 24 hours with 50 ng x mL-1 PMA (ab120297) or PMA with 1 ug x mL-1 LPS for the last 6 hours (duplicates; +/- SD).

    2e6 snap frozen cells were lysed in 0.3 mL RIPA lysis buffer on ice, sonicated in an ice cold water bath for 12 intervals for a total of 3.5 minutes. The supernatants were cleared by centrifugation (max speed, 10 minutes, 4 degrees), and tested undiluted or diluted 1/4.

  • CD86 measured in undiluted 72 hours culture supernatants from control of treated (5 ug x mL-1 PHA; Sigma) PBMCs (1e6 cells x mL-1; duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab45921

プロトコール

Human CD86 ELISA Kit (ab45921) 使用論文

ab45921 has not yet been referenced specifically in any publications.

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