製品の概要

  • 製品名Human CD62L ELISA Kit (Selectin L)
    CD62L キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 15 28ng/ml 1.3 4.6%
    Sample B 19 9ng/ml 0.5 5.5%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1 ng/ml
  • 検出範囲
    1.6 ng/ml - 60 ng/ml
  • 全工程の試験時間
    1h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62L.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein.
  • 別名
    • A.11
    • AI528707
    • CD62 antigen ligand
    • CD62 antigen-like family member L
    • gp90 MEL
    • hLHRc
    • IgA nephropathy, susceptibility to, included
    • L Selectin
    • LAM1
    • LECAM1
    • Leu8
    • Leukocyte adhesion molecule 1
    • Leukocyte surface antigen Leu8
    • Leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Ly-22
    • Ly-m22
    • LYAM1
    • Lymph node homing receptor
    • Lymphocyte adhesion molecule 1
    • Lymphocyte antigen 22
    • Lymphocyte surface MEL-14 antigen
    • PLNHR
    • Selectin L
    • Selectin, lymphocyte
    • SELL
    • TQ1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD62L ELISA Kit (Selectin L) 画像

  • Representative Standard Curve using ab45917

プロトコール

Human CD62L ELISA Kit (Selectin L) (ab45917) 使用論文

This product has been referenced in:
  • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

See 1 Publication for this product

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