製品の概要

  • 製品名Human CD62E ELISA Kit (Selectin)
    CD62E キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 12 31.55ng/ml 0.272 0.86%
    B 12 4.22ng/ml 0.174 4.11%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 32 31.34ng/ml 0.477 1.52%
    B 32 4.18ng/ml 0.266 6.36%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.5 ng/ml
  • 検出範囲
    1 ng/ml - 32 ng/ml
  • 全工程の試験時間
    2h 10m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD62E (Selectin) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD62E in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62E has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62E concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62E are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62E present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62E.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD62 antigen-like family member E
    • CD62E
    • CD62E antigen
    • E selectin
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte endothelial cell adhesion molecule 2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD62E ELISA Kit (Selectin) 画像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • CD62E measured in RIPA-lysates of  control (CTRL) or in HUVEC cells stimulated with LPS (Sigma) for 6 hours (0.5 microgram x mL-1 LPS) or for 24 hours (0.5 microgram x mL-1 LPS for the first 18 hours, 1 microgram x mL-1 for the last 6 hours). Results shown as ng per million cells (duplicates +/- SD). Samples tested at 0.5-20 million lysed cells per mL.

  • CD62E measured in biological fluids (dilution range 1/1-1/20; duplicates +/- SD).

  • Representative Standard Curve using ab45914

プロトコール

Human CD62E ELISA Kit (Selectin) (ab45914) 使用論文

ab45914 has not yet been referenced specifically in any publications.

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