製品の概要

  • 製品名
    Human CD62E ELISA Kit (Selectin)
    CD62E キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 12 31.55ng/ml 0.272 0.86%
    B 12 4.22ng/ml 0.174 4.11%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 32 31.34ng/ml 0.477 1.52%
    B 32 4.18ng/ml 0.266 6.36%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.5 ng/ml
  • 検出範囲
    1 ng/ml - 32 ng/ml
  • 全工程の試験時間
    2h 10m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD62E (Selectin) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD62E in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62E has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62E concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62E are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62E present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62E.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • CD62E measured in RIPA-lysates of  control (CTRL) or in HUVEC cells stimulated with LPS (Sigma) for 6 hours (0.5 microgram x mL-1 LPS) or for 24 hours (0.5 microgram x mL-1 LPS for the first 18 hours, 1 microgram x mL-1 for the last 6 hours). Results shown as ng per million cells (duplicates +/- SD). Samples tested at 0.5-20 million lysed cells per mL.

  • CD62E measured in biological fluids (dilution range 1/1-1/20; duplicates +/- SD).

  • Representative Standard Curve using ab45914

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参考文献

ab45914 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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