製品の概要

  • 製品名Human CD44var ELISA Kit (v6)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8 4.2%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.05 ng/ml
  • 検出範囲
    0.32 ng/ml - 10 ng/ml
  • 添加回収試験

    106 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 106 89% - 115%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD44var (v6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD44var (v6) concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), and urine.

    CD44var (v6) specific antibodies have been precoated onto a 96-well plate. Standards and test samples are added to the wells and along with a CD44var (v6) HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD44var (v6) amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性Contains 1 Link domain.
  • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44_HUMAN
    • CDw44
    • Cell surface glycoprotein CD44
    • Chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • Hematopoietic cell E and L selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • Homing function and Indian blood group system
    • HUTCH-I
    • Hyaluronate receptor
    • IN
    • Indian blood group
    • LHR
    • MC56
    • MDU2
    • MDU3
    • MIC4
    • PGP-1
    • PGP-I
    • Pgp1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD44var ELISA Kit (v6) 画像

  • Representative Standard Curve using ab119519.

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Human CD44var ELISA Kit (v6) (ab119519) 使用論文

ab119519 has not yet been referenced specifically in any publications.

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After reading the protocol I am sure that the whole plate is coated with the antibody as all the reagents are designed to be sufficient for 12 stripes. As mentioned in the protocol, the 32 wells are just an examp...

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