製品の概要

  • 製品名Human CD44 ELISA Kit
    CD44 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 9.85ng/ml 1.55 1.52%
    Sample B 8 10.5ng/ml 0.055 4.76%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 50 9.95ng/ml 0.307 3.08%
    Sample B 50 1.07ng/ml 0.105 9.34%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.13 ng/ml
  • 検出範囲
    0.31 ng/ml - 10 ng/ml
  • 全工程の試験時間
    2h 10m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD44.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性Contains 1 Link domain.
  • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • LHR
    • BA-1
    • CD 44
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44 molecule (Indian blood group)
    • CD44_HUMAN
    • CDw44
    • Cell surface glycoprotein CD44
    • chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • HCELL
    • hematopoietic cell E- and L-selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • homing function and Indian blood group system
    • HSA
    • HUTCH-I
    • HUTCH1
    • Hyaluronate receptor
    • IN
    • MC56
    • MDU2
    • MDU3
    • MGC10468
    • MIC4
    • MUTCH1
    • PGP-1
    • PGP-I
    • PGP1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD44 ELISA Kit 画像

  • huCD44 measured in biofluids showing quantity (ng) per mL of tested sample

プロトコール

Human CD44 ELISA Kit (ab45912) 使用論文

This product has been referenced in:
  • Lau WM  et al. CD44v8-10 is a cancer-specific marker for gastric cancer stem cells. Cancer Res 74:2630-41 (2014). Read more (PubMed: 24618343) »
  • Simpson KL  et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis 4:e613 (2013). ELISA ; Human . Read more (PubMed: 23640455) »

See all 4 Publications for this product

Product Wall

Thank you for your interest in the CD44 and CD56 ELISA kits, ab45912 and ab119587. Neither has been tested for reactivity with mouse or rat samples. We are unable to assess the probability of cross-reaction by BLAST, as the locations of the epitopes th...

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Thank you for contacting Abcam regarding ab45912.


The wash buffer we recommend if you have run out is PBS with 0.05% Tween 20.


I hope this information is helpful. Please do not hesitate to contact us if you have any addition...

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Thank you for the question and for the customer to have taken the time to illustrate the question.

The laboratory confirmed thatboth antibodies will recognize the part after the cleavage site - the part which will be detached from the cells -...

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Thank you for your inquiry and your patience.

Unfortunately the antibody used as detection antibody has not been tested in any other application as IHC or ICC. We do therefore not know whether it can detect the CD44 on the cells.

Th...

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Thank you for your enquiry and your interest. This product has been specifically tested and it is suitable for cell culture supernatant, serum, plasma. We have not tested in other body fluids. For further information, please take a look ...

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Thank you for your enquiry about ab45912. The constitutions of recommended lysis buffer is as follows; NaCl 150mM, NP-40 1%, Tris-HCl 20mM -adjust to pH=8 with protease inhibitors. I hope this information helps you, and please do not hesitate to con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"