Human CD40L ELISA Kit (ab99991)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD40L ELISA Kit
TRAP/CD40L キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
8.23 pg/ml - 6000 pg/ml -
添加回収試験
95 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.35 86% - 105% Serum 93.57 83% - 103% Plasma 94.63 84% - 105% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human CD40L ELISA Kit (ab99991) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40L in serum, plasma (EDTA or heparin), and cell culture supernatants.
This assay employs an antibody specific for Human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human CD40 Ligand ELISA Kit (TNFSF5) (ab196268) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human CD40L 2 vials CD40L Microplate (12 x 8 well strips) 1 unit Recombinant Human CD40L Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
組織特異性
Specifically expressed on activated CD4+ T-lymphocytes. -
関連疾患
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- CD 40L
- CD154
- CD40 antigen ligand
see all -
参照データベース
- Entrez Gene: 959 Human
- Omim: 300386 Human
- SwissProt: P29965 Human
- Unigene: 592244 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab99991 は 3 報の論文で使用されています。
- Herold Z et al. High plasma CD40 ligand level is associated with more advanced stages and worse prognosis in colorectal cancer. World J Clin Cases 10:4084-4096 (2022). PubMed: 35665117
- Kuryk L et al. Quantification and functional evaluation of CD40L production from the adenovirus vector ONCOS-401. Cancer Gene Ther 26:26-31 (2019). PubMed: 30057416
- Garofalo M et al. Heterologous and cross-species tropism of cancer-derived extracellular vesicles. Theranostics 9:5681-5693 (2019). PubMed: 31534511