アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Abcam’s CD31 (PECAM1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium
A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.
This kit will recognize both endogenous and recombinant Human CD31.
Get results in 90 minutes with Human CD31 ELISA Kit (PECAM) (ab190814) from our SimpleStep ELISA® range.
|内容||ラベル||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|Biotinylated anti-CD31 antibody||Red||1 x 400µl||2 x 400µl|
|CD31 Control||Silver||2 vials||4 vials|
|CD31 Microplate (12 x 8 well strips)||1 unit||2 units|
|CD31 Standard||Yellow||2 vials||4 vials|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab45910 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|