製品の概要

  • 製品名Human CD28 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.18 ng/ml
  • 検出範囲
    0.78 ng/ml - 50 ng/ml
  • 添加回収試験

    117 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 105 90% - 130%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD28 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD28 concentrations in cell culture supernatant and serum.

    CD28 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD28 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD28 sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • 組織特異性Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD28
    • CD28 antigen
    • CD28 molecule
    • CD28_HUMAN
    • T-cell-specific surface glycoprotein CD28
    • TP44
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD28 ELISA Kit 画像

  • Representative Standard Curve using ab119514.

Human CD28 ELISA Kit (ab119514) 使用論文

ab119514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"