製品の概要

  • 製品名Human CD23 ELISA Kit
    CD23 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6.3%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    6.8 U/ml
  • 検出範囲
    12.5 U/ml - 400 U/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 93 81% - 120%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD23 concentrations in cell culture supernatant and serum and plasma (EDTA, citrate, heparin).

    CD23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD23 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD23 sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • 配列類似性Contains 1 C-type lectin domain.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt
  • 別名
    • BLAST 2
    • BLAST-2
    • C-type lectin domain family 4 member J
    • CD23
    • CD23 antigen
    • CD23A
    • CLEC4J
    • Fc fragment of IgE low affinity II receptor for (CD23)
    • Fc-epsilon-RII
    • FCE2
    • Fcer2
    • FCER2_HUMAN
    • IGEBF
    • Immunoglobulin E-binding factor
    • Low affinity immunoglobulin epsilon Fc receptor
    • Low affinity immunoglobulin epsilon Fc receptor soluble form
    • Lymphocyte IgE receptor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD23 ELISA Kit 画像

  • Representative Standard Curve using ab119512.

プロトコール

Human CD23 ELISA Kit (ab119512) 使用論文

ab119512 has not yet been referenced specifically in any publications.

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