製品の概要

  • 製品名Human CD21 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 50.25U/ml 2.24 4.45%
    Sample B 8 10.44U/ml 0.46 4.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 51.13U/ml 2.29 4.47%
    Sample B 8 10.76U/ml 0.56 5.2%
  • テスト
    2 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 U/ml
  • 検出範囲
    3.12 U/ml - 100 U/ml
  • 全工程の試験時間
    1h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD21 in Human serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for CD21 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD21 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD21 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD21 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD21.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 ラベル 2 x 96 tests 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml
    Biotinylated anti-CD21 antibody Red 2 x 400µl 1 x 400µl
    CD21 Microplate (12 x 8 well strips) 1 x 2 units 1 unit
    CD21 Standard Yellow 4 vials 2 vials
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 2 x 11ml 1 x 11ml
    Streptavidin HRP 4 x 5µl 2 x 5µl
  • 研究分野
  • 関連性CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.
  • 細胞内局在Type 1 membrane protein
  • 別名
    • C3DR
    • Complement C3d receptor
    • Complement receptor type 2
    • Cr2
    • EBV receptor
    • Epstein Barr virus receptor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD21 ELISA Kit 画像

  • Representative Standard Curve using ab45909.

プロトコール

Human CD21 ELISA Kit (ab45909) 使用論文

ab45909 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45909.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"