製品の概要

  • 製品名
    Human CD137 ELISA Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.9%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    0.2 ng/ml
  • 検出範囲
    0.78 ng/ml - 50 ng/ml
  • 添加回収試験

    90 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD137 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD137 concentrations in cell culture supernatant and serum.

    CD137 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD137 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD137 captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Human CD137 polyclonal antibody 1 x 100µl
    Human CD137 monoclonal antibody coated microplate (12 x 8 wells) 1 unit
    Human CD137 Standard, lyophilized (100 ng/mL on reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能
    Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
  • 組織特異性
    Expressed on the surface of activated T-cells.
  • 配列類似性
    Contains 4 TNFR-Cys repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 別名
    • 4-1BB
    • 4-1BB ligand receptor
    • CD137
    • CD137 antigen
    • CDw137
    • Homolog of mouse 4 1BB
    • ILA
    • Induced by lymphocyte activation (ILA)
    • Interleukin activated receptor homolog of mouse Ly63
    • Receptor protein 4 1BB
    • T-cell antigen 4-1BB homolog
    • T-cell antigen ILA
    • TNFRSF9
    • TNR9_HUMAN
    • Tumor necrosis factor receptor superfamily member 9
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119511.

プロトコール

参考文献

ab119511 has not yet been referenced specifically in any publications.

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