製品の概要

  • 製品名Human CD105 ELISA Kit (Endoglin)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    12.35 pg/ml - 3000 pg/ml
  • 添加回収試験

    > 96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.92 87% - 110%
    Serum 120.1 111% - 130%
    Plasma 116.7 94% - 132%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CD105 (Endoglin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD105 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD105 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD105 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD105 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD105 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.
  • 別名
    • AI528660
    • AI662476
    • CD 105
    • CD105 antigen
    • Cell surface MJ7/18 antigen
    • END
    • Endoglin
    • ENG
    • FLJ41744
    • HHT1
    • ORW
    • ORW1
    • Osler Rendu Weber syndrome 1
    • OTTMUSP00000012838
    • OTTMUSP00000012839
    • RP11 228B15.2
    • RP23 17P12.4
    • S endoglin
    • Transmembrane glycoprotein
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CD105 ELISA Kit (Endoglin) 画像

  • Representative Standard Curve using ab100507.

プロトコール

Human CD105 ELISA Kit (Endoglin) (ab100507) 使用論文

ab100507 has not yet been referenced specifically in any publications.

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Thank you for your inquiry.

ab100507 CD105 human ELISA kit has been validated with plasma and is compatible with EDTA, heparin, and citrate anti-coagulants unless otherwise stated in the Introduction section of the manual. Any one from the ab...

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Thank you for your message and I'm sorry to hear you have been having some difficulties.

Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"