製品の概要

  • 製品名Human CCL28 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    27.4 pg/ml - 20000 pg/ml
  • 添加回収試験

    > 65 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 67.7 59% - 81%
    Serum 70.6 60% - 98%
    Plasma 68.6 59% - 82%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性This protein belongs to the subfamily of small cytokine CC proteins. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. This cytokine displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils and binds to chemokine receptors CCR3 and CCR10. It induces calcium mobilization in a dose-dependent manner. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs.
  • 別名
    • CCK 1
    • CCK1
    • CCL 28
    • Chemokine (C-C motif) ligand 28
    • MEC
    • MGC71902
    • Mucosae associated epithelial chemokine
    • OTTHUMP0000022162
    • SCYA28
    • Small inducible cytokine A28
    • Small inducible cytokine A28 [Precursor]
    • Small inducible cytokine subfamily A (Cys-Cys), member 28
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human CCL28 ELISA Kit 画像

  • Representative Standard Curve using ab99988

  • Representative Standard Curve using ab99988

プロトコール

Human CCL28 ELISA Kit (ab99988) 使用論文

This product has been referenced in:
  • Yoon JH  et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). Read more (PubMed: 25412854) »

See 1 Publication for this product

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