製品の概要

  • 製品名Human Cathepsin D ELISA Kit
  • 検出方法Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    156 pg/ml - 10000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Cathepsin D in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin D in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin D specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin D is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin D amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 関連疾患Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.
  • 配列類似性Belongs to the peptidase A1 family.
  • 細胞内局在Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 別名
    • CATD_HUMAN
    • Cathepsin D heavy chain
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Lysosomal aspartyl protease
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Cathepsin D ELISA Kit 画像

  • Cathepsin D measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD). We recommend to test the human plasma, serum and urine samples in the range of 1:20-1:500, the saliva in the range of 1:100-1:1000 and the cell culture supernatants 1:1-1:1000.

  • Representative Standard Curve using ab119586.

プロトコール

Human Cathepsin D ELISA Kit (ab119586) 使用論文

ab119586 has not yet been referenced specifically in any publications.

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Thank you for your call today and for letting us know about the trouble with this kit.

Please keep me updated about the results with the modifications that we discussed, and let me know if there is anything else that we can do for you.
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Either washing or blotting is effective. In this assay, blotting works the best and it is validated. Please follow the protocol.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"