Human Cardiotrophin 1 ELISA Kit (CTF1) (ab99996)


  • 製品名
    Human Cardiotrophin 1 ELISA Kit (CTF1)
    Cardiotrophin 1 キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    16.46 pg/ml - 12000 pg/ml
  • 添加回収試験

    100 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.2 76% - 112%
    Plasma 101.2 70% - 134%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in plasma and cell culture supernatants. (Human Cardiotrophin 1 concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect the endogenous Human Cardiotrophin 1 in normal serum with ab99996, only in serum spiked with Human Cardiotrophin 1.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム



  • 機能
    Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
  • 組織特異性
    Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
  • 配列類似性
    Belongs to the IL-6 superfamily.
  • 細胞内局在
  • Information by UniProt
  • 別名
    • Cardiophin 1
    • Cardiophin1
    • Cardiotrophin I
    • Cardiotrophin-1
    • CardiotrophinI
    • CT 1
    • CT-1
    • CT1
    • CTF 1
    • Ctf1
    • CTF1_HUMAN
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab99996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Cardiotrophin 1 ELISA Kit (CTF1) 画像

  • Representative Standard Curve using ab99996.

  • Standard curve with background signal subtracted (duplicates; +/- SD).


Human Cardiotrophin 1 ELISA Kit (CTF1) (ab99996) 使用論文

ab99996 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Sorry to reply to in English but my Spanish colleague is currently out of the office and my Spanish is unfortunately not good enough.

The source of theCardiotrophin 1 Human ELISA Kit (ab99996)informed meit isvery a...

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Thank you for your inquiry. After reviewing your standard data, I must say the curve actually looks good (see linear and log-log plots in attachments).  As you can see, the signal strength is strong, the linearity is very nice (≥0.98), the duplicate...

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Thank you very much for your patience. I've finally heard back from the senior scientist, and he has the following response (I've placed it in quotes): "To be honest, I really don’t see an issue with the standard curve as it is giving strong signal...

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