Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992)
製品の概要
- 製品名Human Carcino Embryonic Antigen ELISA Kit (CEA)
Carcino Embryonic Antigen CEA キット 製品一覧 - 検出方法Colorimetric
- サンプルの種類Cell culture supernatant, Serum, Plasma
- アッセイタイプSandwich (quantitative)
- 検出感度< 0.2 ng/ml
- 検出範囲0.343 ng/ml - 250 ng/ml
- 添加回収試験
> 100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 116.7 93% - 136% Serum 145.6 135% - 156% Plasma 103.8 82% - 124% - ステップMultiple steps standard assay
- 種交差性交差種: Human
- 製品の概要
Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.
- 特記事項
Optimization may be required with urine samples.
- アプリケーション適用あり: Sandwich ELISAmore details
- 試験プラットフォームMicroplate
法規制情報
製品の特性
- 保存方法Store at -20°C. Please refer to protocols.
-
内容 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CEA 2 vials CEA Microplate (12 x 8 well strips) 1 unit Recombinant Human CEA Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - 研究分野
- 関連性Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
- 細胞内局在Cell Membrane
-
別名
- Carcinoembryonic antigen
- Carcinoembryonic antigen related cell adhesion molecule 5
- CD66e
see all -
参照データベース
- Entrez Gene: 1048 Human
- SwissProt: P06731 Human
関連製品
- SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab99992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human Carcino Embryonic Antigen ELISA Kit (CEA) 画像
-
Sandwich ELISA - Human CEA (ab99992)CEA measured in biological fluids showing quantity (ng) per mL of tested sample
-
Typical Standard CurveRepresentative Standard Curve using ab99992.
-
Typical Standard CurveRepresentative Standard Curve using ab99992.
プロトコール
Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992) 使用論文
This product has been referenced in:
- Chen S et al. Increased miR-449a expression in colorectal carcinoma tissues is inversely correlated with serum carcinoembryonic antigen. Oncol Lett 7:568-572 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24396489) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"