Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992)

Abreviews (1)Q&A (6)Specific References (3)

製品の概要

  • 製品名
    Human Carcino Embryonic Antigen ELISA Kit (CEA)
    Carcino Embryonic Antigen CEA キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.2 ng/ml
  • 検出範囲
    0.343 ng/ml - 250 ng/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 116.7 93% - 136%
    Serum 145.6 135% - 156%
    Plasma 103.8 82% - 124%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CEA (lyophilized) 2 vials
    CEA Microplate (12 x 8 well strips) 1 unit
    Recombinant Human CEA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
  • 細胞内局在
    Cell Membrane
  • 別名
    • Carcinoembryonic antigen
    • Carcinoembryonic antigen related cell adhesion molecule 5
    • CD66e
    • CD66e antigen
    • CEA
    • CEACAM 5
    • CEACAM5
    • DKFZp781M2392
    • Meconium antigen 100
    see all
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab99992 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Sandwich ELISA - Human CEA (ab99992)
      Sandwich ELISA - Human CEA (ab99992)

      CEA measured in biological fluids showing quantity (ng) per mL of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99992.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99992.

    プロトコール

    参考文献

    This product has been referenced in:
    • Yuan X  et al. Spondin2 is a new prognostic biomarker for lung adenocarcinoma. Oncotarget 8:59324-59332 (2017). Read more (PubMed: 28938639) »
    • Wang W  et al. MDM2 binding protein as a predictor of metastasis and a novel prognostic biomarker in patients with gastric cancer. Oncol Lett 14:6409-6416 (2017). Read more (PubMed: 29422956) »

    See all 3 Publications for this product

    ab99992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    7 reviews or Q&A

    10
    Votes: Highest First

    Dear technical team,

    Our customer purchased ab99992 last November and she opened this kit today to test.
    And she confirmed there are 5μl of item G not 200μl so she would like to know if you have alternative item.
    Please let m...

    Read More

    Thank you for your inquiry.

    I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Str...

    Read More

    CEA concentration of normal or lung cancer sera

     Average Excellent 5/5 (Ease of Use)
    Abreviews
    Difference between normal group and lung adenocarcinoma (ADC) group was very clear.

    But the absolute values measured by ab99992 were remarkably higher than clinical tests in hospital or CRO companies.

    To make matters worse, when we increased the dilution factor (x2, x5, or x10 dilution), the absolute values of CEA also gradually increased.

    Hence this kit can be used for only "relative quantification" studies.
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    Can you tell me which antibody is used as a capture antibody for the CEA ELISA # ab99992 that you market?

    Thank you for your inquiry.

    The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up ...

    Read More

    Sorry for bothering you again, but I was wondering if you happen to know if there is an anti-CEA antibody available in you company that is HRP-conjugated? In the report of a PhD student before me I read that she purchased anti-CEA coated with HRP from ...

    Read More

    Thank you for your new enquiry and continuous interest in our products.

    I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

    However, we have two CEA ELISA ...

    Read More

    is there any information regarding detection of mouse proteinwith this kit?

    Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4...

    Read More

    I am interested in ab99992 but also want to compare it to a new technology I am developing and am wondering if I may also purchase the primary antibody used in that kit although it is not in your catalog.

    At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately. We may in future add the antibody in its own right but for now it can only ...

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    Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?

    Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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