Human Anti-Candida albicans IgG ELISA Kit (ab108715)

製品の概要

  • 製品名Human Anti-Candida albicans IgG ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 8 4.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 20 5.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプIndirect
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Candida albicans IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Candida albicans in Human serum and plasma.

     

    A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.

  • アプリケーション適用あり: Indirect ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab108715 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Indirect ELISA Use at an assay dependent concentration.

    プロトコール

    Human Anti-Candida albicans IgG ELISA Kit (ab108715) 使用論文

    ab108715 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Plasma samples were all diluted 1:100 as suggested in the manual. All other steps were performed according to the instructions.
    This ELISA is very fast (pre-coated wells and no blocking step required), however one has to keep in mind that it does not provide quantitative data. The optical densities of the samples are proportionate to the anti-C.albicans IgG levels in the samples. A positive control, a negative control and a cut-off control are provided in order to check the ELISA is working.
    Username

    Abcam user community

    Verified customer

    投稿 Jul 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"