製品の概要

  • 製品名
    Human c-Kit ELISA Kit (CD117)
    c-Kit キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 5.629ng/ml 0.34 6%
    Sample B 8 1.231ng/ml 0.06 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 5.44ng/ml 0.43 7.9%
    Sample B 8 1.314ng/ml 0.1 7.6%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.095 ng/ml
  • 検出範囲
    0.312 ng/ml - 10 ng/ml
  • 全工程の試験時間
    1h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.

    This kit will recognize both endogenous and recombinant Human c-Kit.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti c-Kit 1 x 0.4ml 2 x 0.4ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    c-Kit Control Silver 2 vials 4 vials
    c-Kit Microplate (12 x 8 well strips) 1 unit 2 units
    c-Kit Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 1 x 2 units 1 x 2 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • 細胞内局在
    Cell Membrane; single-pass type I membrane protein
  • 別名
    • CD117
    • Mast/stem cell growth factor receptor precursor
    • PBT
    • Proto oncogene tyrosine protein kinase Kit
    • SCFR
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab45924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab45924.

プロトコール

参考文献

ab45924 has not yet been referenced specifically in any publications.

レビューと Q&A


The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Thank you for your enquiry.

ab45925 is suitable for Cerebrospinal fluid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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