Human c-Kit ELISA Kit (CD117) (ab45924)
Key features and details
- Sensitivity: 0.095 ng/ml
- Range: 0.312 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human c-Kit ELISA Kit (CD117)
c-Kit キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Sample A 8 5.629ng/ml 0.34 6% Sample B 8 1.231ng/ml 0.06 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Sample A 8 5.44ng/ml 0.43 7.9% Sample B 8 1.314ng/ml 0.1 7.6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.095 ng/ml -
検出範囲
0.312 ng/ml - 10 ng/ml -
全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.
A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.
This kit will recognize both endogenous and recombinant Human c-Kit.
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特記事項
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated anti c-Kit 1 x 0.4ml 2 x 0.4ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml c-Kit Control Silver 2 vials 4 vials c-Kit Microplate (12 x 8 well strips) 1 unit 2 units c-Kit Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 1 x 2 units 1 x 2 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. -
細胞内局在
Cell Membrane; single-pass type I membrane protein -
別名
- CD117
- Mast/stem cell growth factor receptor precursor
- PBT
see all -
参照データベース
- Entrez Gene: 3815 Human
- SwissProt: P10721 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab45924 は 1 報の論文で使用されています。
- Wang X et al. miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance. J Transl Med 20:258 (2022). PubMed: 35672774