Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 42 years) (ab43358)

製品の概要

  • 製品名Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 42 years)
    Breast ライゼート 製品一覧
  • 製品の詳細
    Human breast insoluble fraction
  • 特記事項

    Tissue: Breast Location: Right breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIA T2N0M0 Grade: II Gross findings: Tumor size 4 x 5 x 5 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 6 Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells grown in the enlarged ducts contained comedo-necroses. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. Stromal invasions are not seen. The stroma is desmoplastic and infiltrated by numerous chronic inflammatory cells. The lymph nodes show metastasis (0/6).

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43358: Extraction 1: soluble protein fraction ab43356 Human breast tumour tisue lysate ab43355 Human breast normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab43358 Human breast tumour tissue lysate ab43357 Human breast normal tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 42 years) (ab43358) 使用論文

    ab43358 has not yet been referenced specifically in any publications.

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