Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 39 years) (ab43367)


  • 製品名Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 39 years)
    Breast ライゼート 製品一覧
  • 製品の詳細
    Human breast insoluble fraction
  • 特記事項

    Tissue: Breast Location: Right breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIB T1M0 Grade: II Sex: Female Age: 39 years Gross findings: Tumor size 4 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 10 Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells are arranged as lines and groups invading the stroma. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. Some areas show microinvasions. The stroma is infiltrated by small numbers of chronic inflammatory cells. Necroses are seen, but blood vessel invasion is not seen. The lymph nodes show metastasis (6/10). 


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43367: Extraction 1: soluble protein fraction ab43364 Human Breast Tumor Tissue lysate ab43363 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43367 Human Breast Tumor Tissue lysate ab43365 Human Breast Normal Tissue lysate (matched)


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 39 years) (ab43367) 使用論文

    ab43367 has not yet been referenced specifically in any publications.

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