製品の概要

  • 製品名
    Human Anti-Borrelia burgdorferi IgM ELISA Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Pos. Serum 8 8.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Pos. Serum 14 4.1%
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプ
    Indirect
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).

     

    A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.

  • アプリケーション
    適用あり: Indirect ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA Use at an assay dependent dilution.

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