Human Anti-Bordetella pertussis IgG ELISA Kit (ab108709)

製品の概要

  • 製品名Human Anti-Bordetella pertussis IgG ELISA Kit
    Bordetella pertussis IgG キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Pos. Serum 20 3.9%
    Pos. Serum 24 5.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Pos. Serum 12 4.9%
    Pos. Serum 12 6.1%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプIndirect
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Bordetella pertussis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Bordetella pertussis in Human serum and plasma.

    A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgG sample captured in plate.

  • アプリケーション適用あり: Indirect ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab108709 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Indirect ELISA Use at an assay dependent concentration.

    プロトコール

    Human Anti-Bordetella pertussis IgG ELISA Kit (ab108709) 使用論文

    ab108709 has not yet been referenced specifically in any publications.

    Product Wall

    The pertussis toxin is the entire complex. The amount used for coating is FHA to PT diluted 1:2.

    The plates are coated with two proteins: Pertussis toxin and fillamentous hemagglutinin (FHA).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"