製品の概要

  • 製品名Human BMP7 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97.11 84% - 105%
    Serum 96.2 85% - 104%
    Plasma 95.37 86% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s BMP7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human BMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman BMP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.
  • 別名
    • BMP 7
    • Bone morphogenetic protein 7
    • Eptotermin alfa
    • OP 1
    • OP1
    • Osteogenic protein 1
    • OTTHUMP00000166094
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human BMP7 ELISA Kit 画像

  • Representative Standard Curve using ab99985

  • Representative Standard Curve using ab99985

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Human BMP7 ELISA Kit (ab99985) 使用論文

ab99985 has not yet been referenced specifically in any publications.

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