製品の概要

  • 製品名Human BMP6 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 150 pg/ml
  • 検出範囲
    200 pg/ml - 20000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 93.88 84% - 107%
    Serum 96.53 85% - 104%
    Plasma 94.69 84% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s BMP6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human BMP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.
  • 別名
    • BMP 6
    • Bone morphogenetic protein 6
    • Vegetal related (TGFB related) cytokine
    • Vegetal related growth factor (TGFB related)
    • VG 1 R
    • VG 1 related protein
    • Vg1 related sequence
    • VGR
    • VGR 1
    • VGR1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human BMP6 ELISA Kit 画像

  • Representative standard curve using ab99984

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Human BMP6 ELISA Kit (ab99984) 使用論文

ab99984 has not yet been referenced specifically in any publications.

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