製品の概要

  • 製品名
    Human BMP4 ELISA Kit
    BMP4 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    < 96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.25 85% - 104%
    Serum 92.95 80% - 101%
    Plasma 94.32 82% - 102%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s BMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab99982.

  • Representative Standard Curve using ab99982.

プロトコール

参考文献

This product has been referenced in:
  • Wang X  et al. New association of bone morphogenetic protein 4 concentrations with fat distribution in obesity and Exenatide intervention on it. Lipids Health Dis 16:70 (2017). ELISA ; Human . Read more (PubMed: 28376799) »
  • Gustafson B  et al. BMP4 and BMP Antagonists Regulate Human White and Beige Adipogenesis. Diabetes 64:1670-81 (2015). Read more (PubMed: 25605802) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting us. Both kits ab99982 and ab99983 use the same antibody pairs. The homology between mouse and human BMP4 is 98% and it is therefore likely that these kits will detect mouse BMP4 as well. This has not been tested in house...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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