製品の概要
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製品名Human Betacellulin ELISA Kit (BTC)
Betacellulin キット 製品一覧 -
検出方法Colorimetric
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サンプルの種類Cell culture supernatant, Plasma, Tissue Lysate
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アッセイタイプSandwich (quantitative)
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検出感度< 30 pg/ml
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検出範囲24.69 pg/ml - 18000 pg/ml
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添加回収試験
80 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 74.34 65% - 85% Plasma 78.86 67% - 88% -
ステップMultiple steps standard assay
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種交差性交差種: Mouse, Human
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製品の概要
Abcam’s Betacellulin (BTC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Betacellulin in plasma, and cell culture supernatants. (Human Betacellulin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Betacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Betacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Betacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Betacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human Betacellulin in normal serum with ab99987, only in serum spiked with Human Betacellulin.
Get higher sensitivity in only 90 minutes with Human Betacellulin ELISA Kit (ab189575) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 240X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Betacellulin Microplate (12 x 8 well strips) 1 unit Betacellulin Standard Human (recombinant) 2 vials Biotinylated anti-Human Betacellulin (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
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細胞内局在Cell Membrane and Secreted; extracellular space.
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別名
- Betacellulin precursor
- BTC
- Probetacellulin
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参照データベース
- Entrez Gene: 685 Human
- Entrez Gene: 12223 Mouse
- SwissProt: P35070 Human
- SwissProt: Q05928 Mouse
関連製品
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SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab99987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Typical Standard CurveRepresentative Standard Curve using ab99987.
プロトコール
参考文献
ab99987 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"