アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
|Cell culture supernatant||106.7||90% - 122%|
|Serum||113.1||98% - 116%|
|Plasma||102.8||85% - 117%|
Abcam’s Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA® range.
NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|800X HRP-Streptavidin Concentrate||1 x 200µl|
|Beta Nerve Growth Factor Microplate (12 x 8 wells)||1 unit|
|Beta Nerve Growth Factor Standard Human (recombinant)||2 vials|
|Biotinylated anti-Human Beta Nerve Growth Factor (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99986 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|