Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986)

製品の概要

  • 製品名Human Beta Nerve Growth Factor ELISA Kit (NGFB)
    Beta nerve growth factor キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 14 pg/ml
  • 検出範囲
    6.86 pg/ml - 5000 pg/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 106.7 90% - 122%
    Serum 113.1 98% - 116%
    Plasma 102.8 85% - 117%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA® range.

  • 特記事項

    NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
  • 細胞内局在Secreted.
  • 別名
    • Beta nerve growth factor
    • Beta NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • Nerve growth factor (beta polypeptide)
    • Nerve growth factor beta polypeptide
    • Nerve growth factor beta subunit
    • NGFB
    • NID67
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Beta Nerve Growth Factor ELISA Kit (NGFB) 画像

  • Representative Standard Curve using ab99986.

プロトコール

Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986) 使用論文

This product has been referenced in:
  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). Read more (PubMed: 25347857) »

See 1 Publication for this product

Product Wall

Abreviews
Sera of dromedary camels have been validated for the determination of b-NGF using Abcam human b-NGF ELISA kit (ab99986).
Serum from female dromedaries with suspected high serum b-NGF concentration was pooled. Then this serum was subjected to serial dilution (1:2). The dilution was carried out either in Assay Diluent (1x) provided by the manufacturer, or in pool of camel serum with low (undetectable) serum b-NGF concentration. The dilution has been done the same way as the preparation of the standard described in the protocol of the kit. Then, the assay was performed and optical density values have been plotted against dilution.
Date obtained are in the table below.
Standard Concentration Dilution Standard High CS in AD High CS in low CS
ST 1 0 8 0.08 0.07 0.07
ST 2 20.58 7 0.14 0.17 0.14
ST 3 61.7 6 0.25 0.31 0.26
ST 4 185.2 5 0.54 0.65 0.55
ST 5 555.6 4 1.25 1.35 1.22
ST 6 1667 3 2.35 2.38 2.27
ST 7 5000 2 3.32 3.2 3.15
ST 8 10000 1 3.65 3.57 3.54
In conclusion, b-NGF in camel sera after serial dilution showed an excellent parallelism compared to the standard (recombinant human b-NGF diluted in Assay Diluent). The kit is suitable for the determination of b-NGF in the serum of dromedary camels.


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Verified customer

投稿 Sep 13 2016

The kit is not saliva sensitive

Average Good 4/5 (Ease of Use)
Abreviews
The kit is easy to use,
however its not sensitive to saliva samples
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Abcam user community

Verified customer

投稿 Apr 08 2015

Thank you for contacting us.

As stated on the datasheet, ab99986 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at –80&...

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Thank you for alerting us to this issue.

Do you have your original order number and lot number? Depending on which lot you received, the HRP-streptavidin may have contained 8 ul, so you should try spinning down the vial before opening and che...

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Abreview for Beta NGF Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Pig Cell (bronchioaveolar lavage)
Specification: bronchioaveolar lavage
Amount of Sample: 500 µg
Type of Kit Used: ELISA
Positive control: porcine Hippocampus
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: It is really important to follow the recommended sample and standard volumes.
Abcam response: We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.
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投稿 Aug 07 2012

Thank you for letting me know and thank you for participating in the testing program.

Please do not hesitate to let me know if there is anything I might be able to do for you in the future.

That is good news. Thank you for keeping me informed.

Please do not hesitate to contact us if you need any advice or information.

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I am very pleased to hear you would like to accept our offer and test ab99986 in porcine. This code will give you: $470 VALUE OFF YOUR NEXT ORDERbefore the expiration date. To redeem this offer, please submit an Abreview for porcine and include this co...

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Thank you very much for your interest in ab99986.

To our knowledge, ab99986 has not been tested in porcine sample, but there is a 89% level of homology between human and pig, so there is a good chance that the kit will work. Therefore, I can o...

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Merci de nous avoir contactés.
Nous sommes désolés d'apprendre que le nouveau kit d'ab99986 que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacement gratuit de ce produit : xx . Vou...

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