Human beta 2 Microglobulin ELISA Kit (ab108885)
Key features and details
- Sensitivity: 33 pg/ml
- Range: 0.19 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human beta 2 Microglobulin ELISA Kit
beta 2 Microglobulin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.3% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 33 pg/ml -
検出範囲
0.19 ng/ml - 12.5 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.
A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.
Get results in 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Biotinylated beta 2 Microglobulin Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml beta 2 Microglobulin Microplate (12 x 8 well strips) 1 unit beta 2 Microglobulin Standard 2 vials Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
関連疾患
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
配列類似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻訳後修飾
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
細胞内局在
Secreted. Detected in serum and urine. - Information by UniProt
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別名
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
参照データベース
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
関連製品
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SimpleStep ELISA kits
画像
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108885 は 5 報の論文で使用されています。
- Jiang S et al. Oxidation of protein disulfide bonds by singlet oxygen gives rise to glutathionylated proteins. Redox Biol 38:101822 (2021). PubMed: 33338920
- Makridakis M et al. Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma. Sci Rep 10:4815 (2020). PubMed: 32179759
- Riega-Torres J et al. Beta-2 Microglobulin in Whole Unstimulated Saliva Can Effectively Distinguish Between Sjögren's Syndrome and Non-Autoimmune Sicca Symptoms. Arch Rheumatol 32:284-289 (2017). PubMed: 29900998
- Gao N et al. ß2-Microglobulin participates in development of lung emphysema by inducing lung epithelial cell senescence. Am J Physiol Lung Cell Mol Physiol 312:L669-L677 (2017). PubMed: 28213472
- Rizzo R et al. Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection. J Immunol Res 2016:3890306 (2016). PubMed: 27699182