製品の概要

  • 製品名
    Human beta 2 Microglobulin ELISA Kit
    beta 2 Microglobulin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9%
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.19 ng/ml
  • 検出範囲
    1 ng/ml - 20 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.


    A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.


    Get results in 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated beta 2 Microglobulin Antibody 1 x 120µl
    beta 2 Microglobulin Microplate (12 x 8 well strips) 1 unit
    beta 2 Microglobulin Standard 1 x 40ng
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 関連性
    Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
  • 細胞内局在
    Secreted
  • 別名
    • B2M
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Hdcma22p
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

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  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A



We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (http://www.abcam.com/index...

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Ab108885
DISCOUNT CODE: xxxxx
Expiration date:xxxxxxx

Ab99977
DISCOUNT CODE:xxxxxxxxx
Expiration date: xxxxxxxx

I am very pleased to hear that your customer would like to test ab99977 and ab108885 in human liquo...

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Thank you for your enquiry and your interest in our products.

Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

I would advise you to read through the datasheets as well as the Protocol...

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Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

For our kits:

Regardingab99977:Unf...

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Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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