製品の概要

  • 製品名Human BCA1 ELISA Kit
    BCA1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    1.37 pg/ml - 1000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.62 84% - 105%
    Serum 95.79 82% - 105%
    Plasma 93.68 83% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s BCA1 (BLC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.
  • 別名
    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell chemoattractant
    • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
    • B lymphocyte chemoattractant
    • BCA 1
    • BLC
    • BLR1L
    • C X C motif chemokine 13
    • Chemokine (C X C motif) ligand 13
    • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
    • Chemokine CXC motif ligand 13
    • CXC chemokine BLC
    • CXCL13
    • SCYB13
    • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human BCA1 ELISA Kit 画像

  • Representative Standard Curve using ab99981.

  • Representative Standard Curve using ab99981.

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Human BCA1 ELISA Kit (ab99981) 使用論文

ab99981 has not yet been referenced specifically in any publications.

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