The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-ARFGAP3 antibody (ab1308)
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
ADP ribosylation factor GTPase activating protein 1
ADP ribosylation factor GTPase activating protein 3
ADP-ribosylation factor GTPase-activating protein 3
ARF GAP 3
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
Contains 1 Arf-GAP domain.
Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.