製品の概要

  • 製品名Human APRIL ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.4 ng/ml
  • 検出範囲
    0.78 ng/ml - 50 ng/ml
  • 添加回収試験

    76 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 76 % - %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • 組織特異性Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The precursor is cleaved by furin.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A proliferation-inducing ligand
    • APRIL
    • CD256
    • TALL-2
    • TALL2
    • TNF- and APOL-related leukocyte expressed ligand 2
    • TNF-related death ligand 1
    • TNF13_HUMAN
    • TNFSF13
    • TRDL-1
    • Tumor necrosis factor (ligand) superfamily member 13
    • Tumor necrosis factor ligand superfamily member 13
    • Tumor necrosis factor like protein ZTNF2
    • Tumor necrosis factor related death ligand 1
    • ZTNF2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human APRIL ELISA Kit 画像

  • Representative Standard Curve using ab119505.

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Human APRIL ELISA Kit (ab119505) 使用論文

ab119505 has not yet been referenced specifically in any publications.

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