Human APRIL ELISA Kit (ab119505)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human APRIL ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 7.1% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.4 ng/ml -
検出範囲
0.78 ng/ml - 50 ng/ml -
添加回収試験
76 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 76 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).
APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human APRIL polyclonal antibody 1 x 70µl Human APRIL Standard 2 vials Microplate coated with polyclonal antibody to Human APRIL (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究分野
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機能
Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. -
組織特異性
Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The precursor is cleaved by furin. -
細胞内局在
Secreted. - Information by UniProt
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別名
- A proliferation inducing ligand
- A proliferation-inducing ligand
- APRIL
see all -
参照データベース
- Entrez Gene: 8741 Human
- Omim: 604472 Human
- SwissProt: O75888 Human
- Unigene: 54673 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab119505 は 1 報の論文で使用されています。
- Ortiz-Aljaro P et al. Protein and functional isoform levels and genetic variants of the BAFF and APRIL pathway components in systemic lupus erythematosus. Sci Rep 12:11219 (2022). PubMed: 35780200