製品の概要

  • 製品名
    Human Apolipoprotein E ELISA Kit (APOE)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.9%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Cell Lysate
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.03 µg/ml
  • 検出範囲
    0.06 µg/ml - 1 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein E (APOE) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein E concentrations in Human plasma, serum, cerebrospinal fluid and cell culture samples.

    An Apolipoprotein E specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein E specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein E captured in plate. The kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Apolipoprotein E measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108813

プロトコール

参考文献

This product has been referenced in:
  • Reyes-Soffer G  et al. Effects of PCSK9 Inhibition With Alirocumab on Lipoprotein Metabolism in Healthy Humans. Circulation 135:352-362 (2017). Read more (PubMed: 27986651) »
  • Achariyar TM  et al. Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation. Mol Neurodegener 11:74 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27931262) »

See all 5 Publications for this product

レビューと Q&A

Bravo!

Good Good 4/5 (Ease of Use)
Abreviews
YEAH! we used your product again! the data from this ELISA is always great. thanks
Username

Abcam user community

Verified customer

投稿 Jun 26 2014

WOW!

Good Good 4/5 (Ease of Use)
Abreviews
we used your ELISA kit to detect APOE human serum level. this was our second time using your APOE ELISA kit. we bought your product again because we were satisfied withe the result when we used it last time. the protocol was organized well and easy to understand.
Username

Abcam user community

Verified customer

投稿 Jun 10 2014

GREAT!

Good Good 4/5 (Ease of Use)
Abreviews
We use this ELISA for human serum sample. The results was good and we are gonna publish soon with data using this ELISA.
Username

Miss. seungyun yim

Verified customer

投稿 Jun 03 2014

GREAT!

Good Good 4/5 (Ease of Use)
Abreviews
We use this ELISA for human serum sample. The results was good and we are gonna publish soon with data using this ELISA.
Username

Miss. seungyun yim

Verified customer

投稿 Jun 03 2014

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Abreview for Apolipoprotein E Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Human Serum (Human serum)
Specification: Human serum
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: Brain lysate
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Excellent kit, gave very clean results with human serum ApoE.
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jul 03 2013

Thank you for the clarification. I am not sure why the lysis buffer is failing for you, if you mean the one listed in the protocol:

Cell Lysate: Rinse cells with cold PBS and then scrape the cells
into a tube with 5 ml cold PBS with 0.5...

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Yes, the cocktail that is used by the laboratory that validates this kit is likely to be equivalent to what you have. I recommend using the inhibitor cocktail you have as recommended on the datasheet that comes with it.

Yes, EDTA has trouble going into solution at pH7.2. You will need to add NaOH until the pH gets to 8, and then it typically goes into solution. The safest way is with NaOH pellets but if you have an NaOH solution, you can carefully add that while monit...

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Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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