Human Apolipoprotein CI ELISA Kit (APOCI) (ab108808)

製品の概要

  • 製品名Human Apolipoprotein CI ELISA Kit (APOCI)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.06 µg/ml
  • 検出範囲
    0.063 µg/ml - 4 µg/ml
  • 添加回収試験

    98.5 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma, serum, cell lysates and cell culture supernatants.

    An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • 組織特異性Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • 配列類似性Belongs to the apolipoprotein C1 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Apo-CIB
    • Apo-CIB''
    • ApoC-IB
    • ApoC-IB''
    • APOC1
    • APOC1_HUMAN
    • Apolipoprotein C1
    • Truncated apolipoprotein C-I
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein CI ELISA Kit (APOCI) 画像

  • Representative Standard Curve using ab108808

プロトコール

Human Apolipoprotein CI ELISA Kit (APOCI) (ab108808) 使用論文

ab108808 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can prov...

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I am sorry for the delay in getting back to you.

I can confirm that the antibodies used in this ELISA are polyclonal antibodies raised against the full length Apo CI protein.Theexact epitopes of binding are therefore not known and it is likel...

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Thank you for your call.

I can now confirm that in the kit with the lot number1239351 (amount is lotspecific)each vial contains 2 ug of Apo C-I standard.

We provide 2 vials of Apo C-I standard so the customer can use a fresh vial of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"