Human Apolipoprotein B ELISA Kit (APOB) (ab108807)

製品の概要

  • 製品名Human Apolipoprotein B ELISA Kit (APOB)
    Apolipoprotein B キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Cell Lysate
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.008 µg/ml
  • 検出範囲
    0.008 µg/ml - 0.25 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein B (APOB) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein B concentrations in Human plasma, serum, CSF and cell culture samples.


    An Apolipoprotein B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein B captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Apolipoprotein B ELISA Kit (ab190806) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 関連疾患Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 配列類似性Contains 1 vitellogenin domain.
  • 翻訳後修飾Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 細胞内局在Cytoplasm. Secreted.
  • Information by UniProt
  • 別名
    • Apo B-100
    • Apo B-48
    • APOB
    • ApoB 100
    • ApoB 48
    • APOB_HUMAN
    • Apolipoprotein B
    • Apolipoprotein B 100
    • Apolipoprotein B-48
    • Apolipoprotein B48
    • FLDB
    • LDLCQ4
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein B ELISA Kit (APOB) 画像

  • Apolipoprotein B measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108807

プロトコール

Human Apolipoprotein B ELISA Kit (APOB) (ab108807) 使用論文

ab108807 has not yet been referenced specifically in any publications.

Product Wall

Merci pour votre émail.

Nous n'avons pas besoin de plus de formalités. Je vais mettre en l'envoi du nouveau kit.

Nous sommes désolés que l'ancien kit que vous avez reçu ne fonctionne pas comme attendu. Voici le numéro de commande ...

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Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Me temo que el kit ab108807 no puede distinguir entre las isoformas Apo B48 y Apo B100.

The plate is Corning Costar® 2592. It should fit into most plate washers. The wash buffer contains 0.1% Tween -20.



Q1: Will this kit detect mouse ApoB?
A1: No.

Q2: Is it known whether this kit will detect ApoB48 or ApoB100? Or both?
A2: It has not been determined if this kit will specifically detect ApoB48 or ApoB100.

Thank you for your inquiry and I apologize for the delay in this reply.

The lyophilized powder standard is supplied at 0.75 ug. Reconstitute the 0.75 ug of Apo B Standard with 3 ml of EIA Diluent to generate a solution of 0.25 ug/ml. Any left...

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Hello. The cross reactivity was deternmined by purified protein and plasma samples combination. It is not by single determinants. Please let me know if you have any further questions.



I believe this kit includes 0.75ug of standard. Based on the protocol, this provides you 3 mls of 0.25ug/ml of the highest concentration of standard. This starting concentration is subsequently diluted in 2x serial dilutions to produce the cu...

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Thank you for contacting us. There is no cross-reactivity with mouse for ab108807. Please let me know if I can be of further assistance.


Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"