製品の概要

  • 製品名
    Human Apolipoprotein AII ELISA Kit (APOA2)
    Apolipoprotein AII キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • サンプルの種類
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.004 µg/ml
  • 検出範囲
    0.004 µg/ml - 4 µg/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in cell culture samples, serum and plasma, urine and milk.


    An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.


    Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab108805

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参考文献

This product has been referenced in:
  • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . Read more (PubMed: 28122020) »

See 1 Publication for this product

レビューと Q&A

Abreviews

Sample: Human
Amount of Sample: 0-20 µg
Type of Kit Used: ELISA
Positive control: APOB, APOAII, HDL, LDL
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Kit detects APOB
Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.
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投稿 May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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