製品の概要

  • 製品名Human Apolipoprotein AI ELISA Kit (APOA1)
    Apolipoprotein A I キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    1 µg/ml
  • 検出範囲
    0.625 µg/ml - 20 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    3h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.


    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 組織特異性Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • 関連疾患High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • 配列類似性Belongs to the apolipoprotein A1/A4/E family.
  • 翻訳後修飾Palmitoylated.
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Apo-AI
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human Apolipoprotein AI ELISA Kit (APOA1) 画像

  • Apolipoprotein A1 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108804

プロトコール

Human Apolipoprotein AI ELISA Kit (APOA1) (ab108804) 使用論文

This product has been referenced in:
  • Lin Q  et al. Decreased expression of the APOA1-APOC3-APOA4 gene cluster is associated with risk of Alzheimer's disease. Drug Des Devel Ther 9:5421-31 (2015). ELISA . Read more (PubMed: 26491253) »

See 1 Publication for this product

Product Wall

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Vielen Dank für Ihre Anfrage.


Leider haben wir kein weiteres Kit der Charge GR104136 mehr in unserem Lager (weltweit).

Diese Kits ist normalerweise sehr zuverlässig, aber Sie haben Recht, es gibt eine Komponente, die ni...

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Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Abreview for Apolipoprotein A1 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2 µg
Type of Kit Used: ELISA
Positive control: recombinant apolipoprotein A1
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

投稿 Jan 15 2013


Below is the information for the standard specific to your lot:

Standard Curve: Reconstitute the 120 mg of Apo A-I Standard with 3 ml of Diluent to generate a solution of 40 mg/ml. Allow the standard to sit for 10 minutes with gentle ag...

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I contacted the laboratory, and they responded that the Apo A-1 standard should be in micrograms, not nanograms. The current lot's standard is delivered at 60ug and 3mL of diluent would make the 20ug/ml solution. If you respond with your specific ...

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Vielen Dank für Ihre Anfrage. Ich entschuldige mich für die Wartezeit, ich musste auf nähere Angaben aus dem produzierenden Labor warten.

Ich freue mich Ihnen nun bestätigen zu können, dass beide Standards aus aufgere...

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Thank you for contacting Abcam.

After receiving the customer's complaint and abreview showing cross-reactivity with apoB using the kit ab108804 in May, we retested the kit for cross-reactivity. There was no significant cross-reactivity with ot...

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Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

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Thank you for your inquiry.
I am assuming that you are planning to use ab108804? http://www.abcam.com/index.html?datasheet=108804 http://www.abcam.com/index.html?datasheet=108804.
I can confirm that ab108804 has not been tested with cell cult...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"